A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

A high-quality genome assembly and annotation of Thielaviopsis punctulata DSM102798

Blog Article

Abstract Black scorch disease (BSD), caused by the fungal pathogen Thielaviopsis punctulata (Tp) DSM102798, poses a significant threat to date palm cultivation in the United Arab Emirates (UAE).In this study, Chicago and Hi-C libraries were prepared as input for the Dovetail HiRise pipeline to scaffold the genome of Tp DSM102798.We generated an assembly with a total length of 28.23 Mb comprising 1,256 scaffolds, read more and the assembly had a contig N50 of 18.56 kb, L50 of three, and a BUSCO completeness score of 98.

6% powell and mahoney bloody mary mix for 758 orthologous genes.Annotation of this assembly produced 7,169 genes and 3,501 Gene Ontology (GO) terms.Compared to five other Thielaviopsis genomes, Tp DSM102798 exhibited the highest continuity with a cumulative size of 27.598 Mb for the first seven scaffolds, surpassing the assemblies of all examined strains.These findings offer a foundation for targeted strategies that enhance date palm resistance against BSD, and foster more sustainable and resilient agricultural systems.